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Showing 1–19 of 19 results
Advanced filters: Author: Prisca Liberali Clear advanced filters

  • Live imaging of organoid growth remains a challenge: it requires long-term imaging of several samples simultaneously and dedicated analysis pipelines. Here the authors report an experimental and image processing framework to turn long-term light-sheet imaging of intestinal organoids into digital organoids.

    • Gustavo de Medeiros
    • Raphael Ortiz
    • Prisca Liberali
    ResearchOpen Access
    Nature Communications
    Volume: 13, P: 1-14

  • An organoid-based screening platform maps the genetic interactions underlying intestinal development and regeneration, showing that retinoic acid metabolism maintains the balance between regeneration and homeostasis, and that an antagonist of the retinoid X receptor promotes regeneration in vivo.

    • Ilya Lukonin
    • Denise Serra
    • Prisca Liberali
    Research
    Nature
    Volume: 586, P: 275-280

  • Two major advances in optical pooled screening improve substantially on sensitivity and robustness, expanding its applicability to a broader range of biological contexts.

    • Maurice Kahnwald
    • Marius Mählen
    • Prisca Liberali
    News & Views
    Nature Biotechnology
    Volume: 43, P: 1055-1057

  • Cycler constructs a trajectory of cell-cycle progression from fixed images of cells enabling the correlation of an individual cell's position in the cell cycle with multiple cellular readouts.

    • Gabriele Gut
    • Michelle D Tadmor
    • Prisca Liberali
    Research
    Nature Methods
    Volume: 12, P: 951-954

  • The lack of endogenous reporter lines is a bottleneck in the study of subcellular dynamics in human adult stem cell (ASC)-derived organoids. An approach using CRISPR–Cas9-mediated homology-independent organoid transgenesis (CRISPR–HOT) in ASC-derived organoids now narrows the gap between basic research and translational studies in human organoids.

    • Qiutan Yang
    • Koen C. Oost
    • Prisca Liberali
    News & Views
    Nature Cell Biology
    Volume: 22, P: 261-263

  • Cell biologists must decide whether to embrace the maturing field of systems biology. We argue that a fusion of the two is urgently needed to strengthen both fields.

    • Prisca Liberali
    • Lucas Pelkmans
    Comments & Opinion
    Nature Cell Biology
    Volume: 14, P: 1233

  • The human endoderm-derived organoid cell atlas (HEOCA) presents an integrative analysis of single-cell transcriptomes across different conditions, sources and protocols. It compares cell types and states between models, and harmonizes cell annotations through mapping to primary tissues.

    • Quan Xu
    • Lennard Halle
    • J. Gray Camp
    ResearchOpen Access
    Nature Genetics
    Volume: 57, P: 1201-1212

  • This paper introduces and benchmarks a statistic, the hierarchical interaction score, a statistic for measuring functional interactions between genes from large-scale data, and provides accessible methods for calculating this score.

    • Berend Snijder
    • Prisca Liberali
    • Lucas Pelkmans
    Research
    Nature Methods
    Volume: 10, P: 1089-1092

  • Susceptibility to drug treatment or viral infection can vary greatly from one cell to another even in a population of genetically identical cells cultured together, but until now the causes of this heterogeneity had not been investigated. Here, deterministic links are revealed between fundamental cellular features and a cell's population context — for example, whether a cell is localized at the centre or at the periphery of an island of adhering cells.

    • Berend Snijder
    • Raphael Sacher
    • Lucas Pelkmans
    Research
    Nature
    Volume: 461, P: 520-523

  • Large-scale genetic perturbation screens have been central to many biological discoveries. This Review outlines the recent advances in the quantification of various perturbations across large numbers of single cells simultaneously and describes the use of genetic perturbation screens to infer functional interactions between genes and phenotypes.

    • Prisca Liberali
    • Berend Snijder
    • Lucas Pelkmans
    Reviews
    Nature Reviews Genetics
    Volume: 16, P: 18-32

  • Small intestinal crypts contain twice as many effective stem cells as large intestinal crypts, and this difference is determined by the degree of Wnt-driven retrograde cell movement—which is largely absent in the large intestine—counteracting conveyor-belt-like upward movement.

    • Maria Azkanaz
    • Bernat Corominas-Murtra
    • Jacco van Rheenen
    Research
    Nature
    Volume: 607, P: 548-554

    • Christoph Bock
    • Michael Boutros
    • Robert G. J. Vries
    CorrespondenceOpen Access
    Nature Biotechnology
    Volume: 39, P: 13-17

  • Goblet cells through their production and secretion of mucins play an essential role in protecting the host against luminal insults, and contribute to the mutualism between host and gut microbiota. Loss of epithelial Gpr35 disrupts the goblet cell compartment by reducing their numbers and increasing pyroptosis levels. Dysregulation of goblet cells is correlated with changes in the mucosa-associated bacterial communities. As a result, Gpr35f/fVil+ mice displayed increased susceptibility to C. rodentium-induced colitis. (Adapted from SERVIER MEDICAL ART (CC of license 3.0)).

    • Hassan Melhem
    • Berna Kaya
    • Jan Hendrik Niess
    ResearchOpen Access
    Mucosal Immunology
    Volume: 15, P: 443-458