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Showing 1–17 of 17 results
Advanced filters: Author: Sabine L. Flitsch Clear advanced filters

  • Establishing generic carbohydrate sequencing methods is both a major scientific challenge and a strategic priority. Here the authors show a hybrid analytical approach integrating molecular spectroscopy and mass spectrometry to resolve carbohydrate isomerism, anomeric configuration, regiochemistry and stereochemistry.

    • Baptiste Schindler
    • Loïc Barnes
    • Isabelle Compagnon
    ResearchOpen Access
    Nature Communications
    Volume: 8, P: 1-7

  • Aldehyde-bearing proteins are shown to be suitable substrates for Horner–Wadsworth–Emmons reactions. Applying this process to proteins and glycoproteins enables site-specific bioconjugation with tunable reaction kinetics.

    • Antonio Angelastro
    • Alexey Barkhanskiy
    • Sabine L. Flitsch
    ResearchOpen Access
    Nature Synthesis
    Volume: 3, P: 976-985

  • The vast majority of core structures of protein and peptide glycosylation motifs belong to either O-linked or N-linked glycans. A recent publication describes the structure and biosynthesis of an unusual S-linked glycan linkage in the antibacterial glycopeptide sublancin.

    • Róbert Šardzík
    • Peter Both
    • Sabine L Flitsch
    News & Views
    Nature Chemical Biology
    Volume: 7, P: 69-70

  • Biocatalytic methods to access thioesters, such as acyl-coenzyme A, from carboxylic acids are underdeveloped. Now, it is shown that the adenylation domain of a carboxylic acid reductase enzyme can be exploited as a promiscuous thioester synthetase and combination with acyltransferases facilitates the synthesis of amides and peptide labelling.

    • Christian Schnepel
    • Laura Rodríguez Pérez
    • Sabine L. Flitsch
    Research
    Nature Catalysis
    Volume: 6, P: 89-99

  • In this Primer, Flitsch and colleagues describe how biocatalysis is facilitating synthetic chemistry in both academia and industry. Detailed considerations required to find, select and optimize a biocatalyst are described, followed by an analysis of the performance metrics used to define a good industrial catalyst.

    • Elizabeth L. Bell
    • William Finnigan
    • Sabine L. Flitsch
    Reviews
    Nature Reviews Methods Primers
    Volume: 1, P: 1-21

  • Biocatalytic methods are lacking in current computer-aided synthesis planning tools. Now, RetroBioCat allows computer-aided design of biocatalytic cascades for organic synthesis and synthetic biology. The program was validated using reported cascades and is freely available at retrobiocat.com.

    • William Finnigan
    • Lorna J. Hepworth
    • Nicholas J. Turner
    Research
    Nature Catalysis
    Volume: 4, P: 98-104

  • By employing chemo-enzymatic techniques, a diverse 65-member ganglioside glycan library has been generated that provides a comprehensive insight into ganglioside-mediated cellular communication.

    • Sabine L. Flitsch
    • Josef Voglmeir
    News & Views
    Nature Chemistry
    Volume: 16, P: 843-844

  • The biological functions of glycan motifs such as the Lewis blood antigens are often defined by their precise multivalent presentation on complex glycoconjugates, making synthesis particularly challenging. Access to a number of positionally defined Lewis motifs on natural polysaccharide scaffolds has now been achieved using bacterial glycosyltransferases.

    • Kun Huang
    • Sabine L Flitsch
    News & Views
    Nature Catalysis
    Volume: 2, P: 479-480

  • Nature has a whole battery of dedicated enzymes to make the complex links between sugar rings — how can synthetic chemists compete? An ingenious approach fills a big gap in the synthetic tool-kit.

    • Sabine L. Flitsch
    News & Views
    Nature
    Volume: 437, P: 201-202

  • Distinguishing between different proteins that each bind to the same type of glycan is challenging. Here, the authors demonstrate that an enzymatically synthesised library of Lewisx ‘glycofluoroforms’ that feature site-specific fluorination can discriminate closely related proteins.

    • Kristian Hollingsworth
    • Antonio Di Maio
    • Bruno Linclau
    ResearchOpen Access
    Nature Communications
    Volume: 15, P: 1-14

  • The interactions of sugars and proteins underlie many biological processes, and cataloguing them is a daunting task. A technique for attaching sugars to microarrays offers a promising, high-throughput solution.

    • Sabine L. Flitsch
    • Rein V Ulijn
    News & Views
    Nature
    Volume: 421, P: 219-220

  • Changes in glycoprotein expression are correlates of disease, but secreted glycoproteins cannot be accurately traced to their cell line of origin. Here, the authors develop a strategy to chemically tag and profile glycoproteins in a cell line-specific manner in co-culture systems and in vivo.

    • Anna Cioce
    • Beatriz Calle
    • Benjamin Schumann
    ResearchOpen Access
    Nature Communications
    Volume: 13, P: 1-18

  • Identification of glycosylation patterns is complicated by the lack of sensitive analytical techniques that can distinguish between epimeric carbohydrates. It has now been shown that ion-mobility tandem mass spectrometry of ions derived from glycopeptides and oligosaccharides enables glycan stereochemistry to be determined, highlighting the potential of this technique for sequencing complex carbohydrates on cell surfaces.

    • P. Both
    • A. P. Green
    • C. E. Eyers
    Research
    Nature Chemistry
    Volume: 6, P: 65-74

  • Engineering enzymes capable of performing chemical transformations requires high-throughput assays to screen activity. In this protocol, reactions in cell lysates are analyzed directly by desorption electrospray ionization (DESI) mass spectrometry.

    • Ruth Knox
    • Rachel Smith
    • Perdita E. Barran
    Protocols
    Nature Protocols
    Volume: 20, P: 3295-3313