The authors found that size selection magnetic beads could remove long RNA transcripts from total RNA with simplistic operation. After long transcript removal, microRNA could be concentrated and efficiently reverse-transcribed by stem-loop-6N primer. Touchdown qPCR improved microRNA quantification with lower CT values and better detection efficiency. Finally, they incorporated these observations and created a new protocol named long transcripts minus touchdown qPCR for screening and quantifying microRNAs.
- Xi Wang
- Yixiao Feng
- Zongyue Zeng
