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  • This protocol for CRISPR–Cas9 editing in primary mouse T cells enables studies of gene-edited CAR T cells in immune-intact cancer models, enabling users to explore the impact of a given gene deletion on CAR T cells within a natural tumor microenvironment.

    • Puneeth Guruprasad
    • Ranjani Ramasubramanian
    • Marco Ruella
    Protocols
    Nature Protocols
    Volume: 20, P: 3629-3654