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Nature Chemical Biology (2)
Nature Methods (1)

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Showing 1–3 of 3 results

Advanced filters: Author: Steven De Gieter Clear advanced filters

MISO: microfluidic protein isolation enables single-particle cryo-EM structure determination from a single cell colony

The micro isolation (MISO) method combines microfluidics-based protein purification with cryo-EM grid preparation, enabling cryo-EM structure determination of soluble and membrane proteins starting from less than 1 µg of a target protein.

Gangadhar Eluru
Steven De Gieter
Rouslan G. Efremov
Research13 Nov 2025
Nature Methods

Volume: 22, P: 2563-2573
An intrinsically disordered entropic switch determines allostery in Phd–Doc regulation

Crystal structure analysis of the Phd transcription factor bound to the DNA operator combined with biophysical studies reveal an interplay between intrinsically disordered regions and conditional cooperativity for operon binding and repression.

Abel Garcia-Pino
Steven De Gieter
Remy Loris
Research02 May 2016
Nature Chemical Biology

Volume: 12, P: 490-496
The Fic protein Doc uses an inverted substrate to phosphorylate and inactivate EF-Tu

The Doc-Phd toxin-antitoxin system inhibits bacterial translation via an unknown mechanism. Functional and structural analyses now show that Doc, which has an active site like AMPylating Fic proteins, actually works as a kinase, phosphorylating EF-Tu to block translation.

Daniel Castro-Roa
Abel Garcia-Pino
Nikolay Zenkin
Research20 Oct 2013
Nature Chemical Biology

Volume: 9, P: 811-817