Steroidal C12β/15α-hydroxylations are essential in synthesizing steroid drugs but remain challenging via chemical and biological methods. Here, the authors engineer the fungal P450 monooxygenase CYP68J5_fg and obtain two variants with high selectivity for C12β- and C15α- hydroxylation of progesterone, alongside enhanced catalytic efficiency.
- Ling Wang
- Ying Wang
- Aitao Li
