The newly transcribed RNA-bound proteome (or RNA interactome) is captured using this protocol by time-dependent incorporation of 5-ethynyluridine into newly transcribed RNAs followed by UV-mediated RNA-protein cross-linking, click-chemistry-aided biotinylation and affinity isolation of RNA–protein complexes.
- Xiangpeng Guo
- Muqddas Tariq
- Miguel A. Esteban
