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The authors reveal that the ferroelectric to antiferroelectric phase transition in Pb(Zr_0.97Ti_0.03)O₃-1wt%Nb₂O₅ at low temperatures is an irreversible process exhibiting relaxation behavior and diffusional kinetics.

TMexCD1-TOprJ1 is the paradigm for transferable tigecycline resistance by efflux pumps. Here, authors report biochemical and cryo-EM structural description of TMexCD1-TOprJ1 tripartite machinery, illuminating its assembly and inhibition mechanism. It benefits developing next-generation of efflux pump-targeted antimicrobials.

The endoplasmic/sarcoplasmic reticulum (ER/SR) plays a crucial role in calcium signaling, but there are few methods capable of efficiently capturing ER/SR Ca²⁺ dynamics. Here authors develop a set of genetically encoded indicators enabling ratiometric super-resolution imaging and detection of elementary Ca²⁺ release in cardiomyocytes.

STIM proteins are key regulators of intracellular Ca²⁺ signalling. Here, Wang et al. demonstrate that subtle differences between STIM1 and its close homologue STIM2 have profound consequences for their ability to gate Orai1 Ca²⁺channels, thus revealing the basis for their distinct physiological functions.

Here the authors identify a role of GPAT4 in endocardial and myocardial development through maintaining ER homeostasis. GPAT4 deficiency triggers ER stress, ER-mitochondrial dysfunction, and activation of the cGAS-STING-type I IFN response, leading to severe heart malformation.

Wang et al. show how glucose sensing via O-GlcNAcylation drives the assembly of a glycolytic metabolon on the mitochondrial surface to couple metabolic efficiency with neuronal activity.

The human tumor suppressor p53 interacts with the BCL-2 family proteins to regulate apoptosis. Here, the authors solve the structures of p53 in complex with the antiapoptotic protein BCL-2 and suggest a mechanism by which p53 promotes apoptosis by competitively antagonizing the interaction of BCL-2 with pro-apoptotic BCL-2 family proteins.

The NEMO series of genetically encoded calcium indicators report calcium activity in neuronal and non-neuronal cells with high signal-to-baseline ratio, which is shown in neuronal culture, slice preparations, in vivo and in planta.

A small molecule, CGI1746, was identified to block ferroptosis acting through the sigma-1 receptor, a chaperone primarily at endoplasmic reticulum–mitochondria contacts to mediate calcium transfer.

While the shortwave infrared (SWIR) region has potential for bioimaging applications, stable and biocompatible SWIR absorbers/emitters are challenging to access. Here, the authors report the approach of crystal-aided aggregate synthesis for the development of J-aggregates for in vivo bioimaging.

Wild teas are considered as valuable resource for studying domestication and breeding. Here, Zhang et al. report genome of wild tea DASZ and transcriptome of 217 accessions, which clarify pedigree of Chinese tea cultivars and show tea may not have undergone long-term artificial directional selection on flavor-related metabolites.

Here the authors use cryo-electron microscopy to provide insights into fatty acid recycling by an acyl-acyl carrier protein (ACP) synthesizing enzyme, showing a transition from a hexameric to tetrameric form in the process of acyl-ACP production.

Radial-velocity measurements of a Galactic B-type star show a dark companion that seems to be a black hole of about 68 solar masses, in a widely spaced binary system.

Existing optogenetic methods to induce calcium mobilisation lack selectivity and specificity. Here, the authors design and engineer a single-component light-operated calcium channel to provide optical control over calcium signals and calcium-dependent physiological responses: LOCa.

How plasma membrane Orai Ca²⁺ channels are activated by STIM proteins to activate Ca²⁺signals is still not fully known. Here the authors show that a nexus region located at the Orai1 C-terminus allows channel gating without a direct interaction of STIM1 with the channel pore.

STIM1, the endoplasmic reticulum calcium sensor, is activated in response to calcium store depletion and translocates to the ER–plasma membrane junctions to activate Orai1 channels. Here the authors present a new model of unimolecular coupling between STIM1 and Orai1 whereby STIM1 dimers may be involved in crosslinking Orai1 channels.

Determining the triboelectric charge and energy density of dielectric materials is generally limited by many factors, failing to reflect their intrinsic behaviour. Here, a standardized strategy is proposed employing contact-separation TENG and supressing air-breakdown to assess max triboelectric charge and energy densities leading to an updated triboelectric series.

Depletion of calcium from intracellular stores induces interaction between the endoplasmic reticulum STIM1 protein and the plasma membrane ORAI1 channel that facilitates cellular calcium entry. Here Ma et al. characterize a STIM1 gain-of-function mutant and propose a conformational switch that controls ORAI1 gating.

Dual-specificity phosphatases regulate several processes associated with carcinogenesis. Here the authors show that inhibition of the dual-specificity phosphatase DUSP18 improves the activity of tumor-infiltrating CD8 T cells, enhancing response to immune checkpoint blockade in preclinical models of colorectal cancer.

Using a sample of more than 2,600 classical Cepheids with precise distances and ages, Huang et al. have discovered that the Milky Way’s disk warp undergoes retrograde precession, revealing the presence of a slightly oblate dark matter halo.

Aberrant and persistent activation of the transcription factor STAT3 has been found in various types of cancers. Here the authors identify TRIM27 as a positive regulator of IL-6-induced STAT3 activation through the formation of JAK1-STAT3 complex, thus impacting inflammation-induced colon cancer development.

The intermembrane complexes mediating calcium signals are crucial but poorly understood. Here the authors identify a Phe-His pair in the calcium-sensing STIM1 protein that controls both activation and pairing of STIM1 with Orai channels to generate calcium signals

STIM proteins are ubiquitous endoplasmic reticulum Ca²⁺ sensors that rapidly translocate to couple with 'store-operated' Orai Ca²⁺ channels when luminal Ca²⁺ levels are low. STIM1 also senses heat changes, which trigger a similar translocation and prime STIM1 to activate Orai, suggesting that STIM1 functions as a sensor of multiple stress signals.

Bacillus thuringiensis (Bt) is an important bioinsecticide, but high-level resistance has been rapidly evolving in agricultural pests. Here, Guo et al. show that the MAPK cascade can be activated by enhanced upstream insect hormone signals to counter Bt virulence in the diamondback moth.

Plutella xylostella endures Bt toxins with no performance costs. This study reveals how, depending on the presence of the toxin, this insect modifies MAPK phosphorylation to modulate the transcription factor FTZ-F1 binding, to up- or down- regulate Bt receptors or non-receptor (resistant) paralogs.

Preparation of xanthene-containing compounds has been limited due to structural bias existing methods pose. Here, the authors developed a mild, diversity-oriented method for rhodamines synthesis, leading to the finding of compounds with antibacterial potency against a variety of bacterial species.

Hydrogen-rich superhydrides are promising high-temperature superconductors which have been observed only at pressures above 170 GPa. Here the authors show that CeH₉ can be synthesized at 80-100 GPa with laser heating, and is characterized by a clathrate structure with a dense 3-dimensional atomic hydrogen sublattice.

Stromal interaction molecule 1 (STIM1) monitors ER-luminal Ca²⁺ levels to maintain cellular Ca²⁺ balance. Here the authors find that the STIM1 luminal domain monomer has multiple Ca²⁺ - binding sites which set the threshold for physiological activation of STIM1 in cells.

Through a proteomic analysis of ER–PM junctions, Zhou and colleagues and Wang and colleagues discover that the transmembrane protein STIMATE is a positive regulator of STIM localization and function, thereby stimulating Ca²⁺ influx.

Functional characterization of preferential and potent small molecule modulators that allosterically enhance the gating of the Otop1 channel by targeting its key gating apparatus