The last step in autophagy involves fusion of autophagosomes with lysosomes to generate autolysosomes. Through proteomics analysis and an RNAi screen, Yu and colleagues provide mechanistic insight into how lysosomes are regenerated from autolysosomes. Their analyses reveal a key role for clathrin and phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2) in this process.
- Yueguang Rong
- Mei Liu
- Li Yu