Building on their recent work establishing long-term cultures from Lgr5+ cells of the small intestine and stomach, Shiro Yui and colleagues describe a new colonic stem cell culture and expansion method generating organoids from single Lgr5+ stem cells. The study provides proof of principle that the cultured Lgr5+ cells can be used for stem cell therapy to repair superficially damaged epithelium in a dextran sulfate sodium (DSS)-induced acute colitis mouse model.
- Shiro Yui
- Tetsuya Nakamura
- Mamoru Watanabe
