In this study, the authors report the identification, biochemical characterization and cryo-electron microscopy structure of a phage-encoded miniature type V CRISPR-Cas nuclease in different functional states. This work provides mechanistic insight into CRISPR-Cas12 nucleases and a solid framework for future rational engineering.
- Satoshi N. Omura
- Lauren E. Alfonse
- Osamu Nureki
