Antibody generation articles from across Nature Portfolio

A bispecific nanobody (BsNb-ncAA) with sites found by computation to improve binding, inhibit immune evasion, boost CD8⁺ T cells, and suppress colon tumor growth, offering promising colorectal cancer immunotherapy strategies.

The burden of herpes simplex virus (HSV) infection is exacerbated due to the emergence of antiviral drug resistance and a lack of effective antibodies. Here the authors construct a bispecific nanobody that blocks receptor binding and prevents gD-gH/gL interaction, and show that it protects against HSV-1 and HSV-2 infection in mice.

Using conditional mutants of AID based on auxin-inducible degron system (AID2 cells), controlled immunoglobulin gene rearrangements in the B cell line DT40 facilitate the generation and stabilization of antigen-specific clones.

Poorly performing antibodies contribute to the reproducibility crisis in biomedical research. The market offers millions of antibodies and validating them is a cumbersome process. Now, the non-profit Antibody Characterization through Open Science (YCharOS) organization shares a step-by-step protocol for rigorous antibody validation using genetic (knockout) cell line controls.

A novel bright near-infrared fluorescent protein inserted into a nanobody enables visualization of native proteins inside living cells and specific manipulation of cell function, including Boolean protein-based operators.

A yeast cell culture approach mimics the benefits of somatic hypermutation to accelerate the discovery of desirable antibodies.