Articles

By assessing different clinical, behavioral and welfare parameters, the authors demonstrate that the oral administration of tramadol is more suitable than metamizole as on-demand analgesia in mouse models of acute lymphoblastic leukemia.

Jovanovic et al. show that a gel-based diet can be used as a minimally invasive alternative system for the delivery of small molecules for microglia depletion and inducible Cre recombination in mice.

The authors report the development of a new rat model of forelimb ischemia–reperfusion injury, characterized by motor coordination deficits, impairment of neuromuscular transmission and serum biochemical and inflammatory changes.

The study reveals that long-term anesthesia with ketamine–xylazine can induce hypoxia and mortality in mice, whereas oxygen supplementation mitigates these effects. As an alternative, isoflurane showed superior safety and fewer effects on the immune system.

The authors describe the second generation of the mutant methionyl tRNA synthetase mouse line, designed to increase mutant enzyme expression, enhance the labeling of nascent proteins and facilitate cell type-specific proteome identification.

The Article describes a minipig model of obesity-associated meta-inflammation, characterized by increased C-reactive protein levels and white blood cells, together with changes in the expression of genes related to inflammation and metabolism.

A new color vision optomotor response assay was evaluated for assessing red cone ablation in transgenic zebrafish. The results suggest that the assay can be useful for evaluating visual function and color vision in zebrafish with visual disorders.

This study uses a transgenic mouse line expressing Cre-inducible astro-hM3Dq to study astrocytes’ role in neuroinflammation and cognition without the use of viral transduction, mitigating the potential side effects associated with standard techniques.

This study analyzes the course of murine astrovirus infection and the immune response in multiple mouse strains to identify the best sentinel strain and the best diagnostic method when including murine astrovirus in health monitoring programs.

The authors compare the efficacy of injectable and oral broad-spectrum antibiotics on reducing bacteria from the upper respiratory tract of ferrets, before evaluating the effects of commensal bacteria reduction on influenza A virus infection.

The authors evaluate multiple factors to improve knock-in rates using CRISPR-mediated homology-directed repair in zebrafish, before demonstrating that prime editing is a superior alternative.

This Article presents the results of a global multi-laboratory study assessing the effects of housing conditions and experimental settings on novel tank test responses in adult zebrafish.

This study, which establishes reference intervals for biochemical and hematological parameters in healthy macaques and describes changes in these parameters after vaccination, will facilitate toxicity monitoring in preclinical vaccine studies.

This proof-of-concept study for the intranasal delivery of neuroactive drugs in adult zebrafish introduces a method that enables fast delivery of drugs such as arecoline and nicotine to the brain while requiring smaller quantities than water immersion methods.

This article describes a computational model to forecast population dynamics in a laboratory colony of common marmosets. The model can be used to predict future animal stock levels and support colony management.

The study shows that, compared with lean animals, obesity significantly impacts echocardiographic findings, infarct size, circulating biomarkers, mitochondrial respiratory capacity and mortality in a minipig model of myocardial infarction.

The authors used an implantable electrophysiological device to assess the effects of two commonly used anesthetics (isoflurane and pentobarbital) on supraventricular electrophysiology and arrhythmic substrate in male and female rats.

The authors compare muscle function, histology and molecular markers, and biochemical plasma profiles in male and female HSA^LR mice to identify potential sex differences in this mouse model of myotonic dystrophy type I.

Wound healing assays are commonly used to study tissue repair in mice. The authors show that wound localization and housing conditions are critical factors that can affect the reliability and reproducibility of the results of this assay.

This study explores the use of electrical stunning as a viable alternative to anesthetic overdose and rapid cooling for euthanizing zebrafish larvae, potentially improving the welfare of over 5 million zebrafish in biomedical research.