This study describes the segregated neural representations at single cell level and in whole brain when mice are presented with positive and negative emotional stimuli given in succession. Using a newly developed technique called tyramide-amplified-immunohistochemistry–fluorescence in situ hybridization (TAI-FISH) to label multiple neuronal populations, the authors demonstrate specific overlap and divergence of neuronal activation pattern to different emotional stimuli.
- Jianbo Xiu
- Qi Zhang
- Hailan Hu
