L-DNase II activation by the 24 kDa apoptotic protease (AP24) in TNFα-induced apoptosis

Altairac, Séverine; Wright, Susan C; Courtois, Yves; Torriglia, Alicia

doi:10.1038/sj.cdd.4401293

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Published: 22 August 2003

L-DNase II activation by the 24 kDa apoptotic protease (AP24) in TNFα-induced apoptosis

Séverine Altairac¹,
Susan C Wright²,
Yves Courtois¹ &
…
Alicia Torriglia¹

Cell Death & Differentiation volume 10, pages 1109–1111 (2003)Cite this article

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Dear Editor,

The 24 kDa apoptotic protease (AP24) is a serine protease with elastase-like activity that is activated during TNFα- or UV light-induced apoptosis.¹ In vitro, it stimulates internucleosomal DNA fragmentation in isolated nuclei. This distinguishes AP24 from several other proteases, such as granzyme B and caspases, which require the presence of cytosolic components to activate nuclear DNA fragmentation in cell-free systems. This feature leads to the hypothesis that AP24 may play a role in transmitting apoptotic signals from the cytosol to the nucleus, where it may directly or indirectly activate endogenous endonucleases, resulting in DNA digestion.

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Acknowledgements

We acknowledge Dr. Anne-Marie Faussat for help in FACS analysis and Dr. Justine R Smith for editing the English manuscript. This work was supported by Association pour la Recherche contre le Cancer (ARC) and Retina France. SA was supported by the Association de Amis des Sciences fellowship.

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Développement, Pathologie et Vieillissement de la Rétine, INSERM U450, Association Claude Bernard, Institut Biomédical des Cordeliers, 15, rue de l'Ecole de Médecine, Paris, France
Séverine Altairac, Yves Courtois & Alicia Torriglia
Palo Alto Institute of Molecular Medicine, Mountain View, CA, USA
Susan C Wright

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Séverine Altairac
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Susan C Wright
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Yves Courtois
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Alicia Torriglia
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Correspondence to Alicia Torriglia.

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Altairac, S., Wright, S., Courtois, Y. et al. L-DNase II activation by the 24 kDa apoptotic protease (AP24) in TNFα-induced apoptosis. Cell Death Differ 10, 1109–1111 (2003). https://doi.org/10.1038/sj.cdd.4401293

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Published: 22 August 2003
Issue date: 01 September 2003
DOI: https://doi.org/10.1038/sj.cdd.4401293

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Increase in the expression of leukocyte elastase inhibitor during wound healing in corneal endothelial cells

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About this article

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This article is cited by

Increase in the expression of leukocyte elastase inhibitor during wound healing in corneal endothelial cells

Apoptosis-inducing factor (AIF) and leukocyte elastase inhibitor/l-DNase II (LEI/LDNaseII), can interact to conduct caspase-independent cell death

Non-caspase proteases: triggers or amplifiers of apoptosis?

Caspase-activated DNase (CAD)-independent oligonucleosomal DNA fragmentation in chronic myeloid leukaemia cells; a requirement for serine protease and Mn2+-dependent acidic endonuclease activity

A novel paraptosis pathway involving LEI/L-DNaseII for EGF-induced cell death in somato-lactotrope pituitary cells

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