Abstract
Calmodulin (CaM) influences many cellular processes by interacting with various proteins. Here, we isolated AtBAG6, an Arabidopsis CaM-binding protein that contains a central BCL-2-associated athanogene (BAG) domain. In yeast and plants, overexpression of AtBAG6 induced cell death phenotypes consistent with programmed cell death (PCD). Recombinant AtBAG6 had higher affinity for CaM in the absence of free Ca2 + than in its presence. An IQ motif (IQXXXRGXXXR, where X denotes any amino-acid) was required for Ca2 +-independent CaM complex formation and single amino-acid changes within this motif abrogated both AtBAG6-activated CaM-binding and cell death in yeast and plants. A 134-amino-acid stretch, encompassing both the IQ motif and BAG domain, was sufficient to induce cell death. Agents generating oxygen radicals, which are known to be involved in plant PCD, specifically induced the AtBAG6 transcript. Collectively, these results suggest that AtBAG6 is a stress-upregulated CaM-binding protein involved in plant PCD.
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Accession codes
Abbreviations
- CaM:
-
calmodulin
- CaMBPs:
-
CaM-binding proteins
- BAG:
-
BCL-2-associated athanogene
- AtBAG:
-
Arabidopsis thaliana BAG
- CDD:
-
cell death domain
- GST:
-
glutathione S-transferase
- HRP:
-
horse radish peroxidase
- PCD:
-
programmed cell death
- ROS:
-
reactive oxygen species
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Acknowledgements
This research was partially supported by grants (PF0330401-00) from the Plant Diversity Research Center of the 21st Century Frontier Research Program, MOST, the Environmental Biotechnology Core Research Center (R15-2003-012-01002-0) from KOSEF/MOST, KRIBB Research initiative program, and Bigreen 21 program, Rural Development Administration, Korea. CH Kang, WY Jung, JC Jeong, DW Baek were supported by scholarships from the Brain Korea 21 program, Ministry of Education, Korea.
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Kang, C., Jung, W., Kang, Y. et al. AtBAG6, a novel calmodulin-binding protein, induces programmed cell death in yeast and plants. Cell Death Differ 13, 84–95 (2006). https://doi.org/10.1038/sj.cdd.4401712
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DOI: https://doi.org/10.1038/sj.cdd.4401712
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