Overexpression of RARγ increases death of SH-SY5Y neuroblastoma cells in response to retinoic acid but not fenretinide

Goranov, B B; Campbell Hewson, Q D; Pearson, A D J; Redfern, C P F

doi:10.1038/sj.cdd.4401824

Letter to the Editor
Published: 09 December 2005

Overexpression of RARγ increases death of SH-SY5Y neuroblastoma cells in response to retinoic acid but not fenretinide

B B Goranov¹,
Q D Campbell Hewson¹,
A D J Pearson¹ &
…
C P F Redfern¹

Cell Death & Differentiation volume 13, pages 676–679 (2006)Cite this article

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Dear Editor,

The retinoic acid receptors (RARs) have an important role in cell differentiation and death. Retinoic acid induces differentiation of neuroblastoma cells in vitro, a property that has led to the successful use of 13-cis retinoic acid, a prodrug for all-trans retinoic acid,¹ in the treatment of minimum residual disease of neuroblastoma patients.² All three RAR subtypes (α, β and γ) can be activated by all-trans or 9-cis retinoic acid, and these receptors function as heterodimers with retinoid-X receptors (RXRs) to enhance or drive the expression of target genes.³ Unlike the all-trans isomer, 9-cis retinoic acid activates both RARs and RXRs. Retinoic acid analogues (retinoids) with a narrower selectivity for the RAR subtypes are undergoing clinical and laboratory evaluation. One such retinoid is fenretinide (n-(4-hydroxyphenyl) retinamide), which is RARβ- and RARγ-selective. Unlike retinoic acid, fenretinide is able to induce apoptosis in a range of neuroblastoma cell lines, including some which are resistant to retinoic acid.⁴ This makes fenretinide a potentially powerful agent for the treatment of neuroblastoma.

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Acknowledgements

We thank Marco Ranalli for instruction in flow cytometry, Penny Lovat for help with cell culture, Gerry Melino for support and encouragement, Serafina Oliverio for a GADD153 plasmid used to optimise the doxycycline treatment, Robert Ross for valuable discussions of neuroblastoma cell morphology, Uwe Reichert and Galderma for the RAR antagonists, and the North of England Children's Cancer Research Fund and the Newcastle Hospitals Joint Research Executive for funding.

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Northern Institute for Cancer Research, Paul O'Gorman Building, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne, NE2 4HH, UK
B B Goranov, Q D Campbell Hewson, A D J Pearson & C P F Redfern

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B B Goranov
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Q D Campbell Hewson
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A D J Pearson
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C P F Redfern
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Correspondence to C P F Redfern.

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Goranov, B., Campbell Hewson, Q., Pearson, A. et al. Overexpression of RARγ increases death of SH-SY5Y neuroblastoma cells in response to retinoic acid but not fenretinide. Cell Death Differ 13, 676–679 (2006). https://doi.org/10.1038/sj.cdd.4401824

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Published: 09 December 2005
Issue date: 01 April 2006
DOI: https://doi.org/10.1038/sj.cdd.4401824

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Enhancing Neurological Competence of Nanoencapsulated Cordyceps/Turmeric Extracts in Human Neuroblastoma SH-SY5Y Cells

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Enhancing Neurological Competence of Nanoencapsulated Cordyceps/Turmeric Extracts in Human Neuroblastoma SH-SY5Y Cells

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