Abstract
Protein synthesis is inhibited during apoptosis. However, the translation of many mRNAs still proceeds driven by internal ribosome entry sites (IRESs). Here we show that the 5′UTR of hid and grim mRNAs promote translation of uncapped-mRNA reporters in cell-free embryonic extracts and that hid and grim mRNA 5′UTRs drive IRES-mediated translation. The translation of capped-reporters proceeds in the presence of cap competitor and in extracts where cap-dependent translation is impaired. We show that the endogenous hid and grim mRNAs are present in polysomes of heat-shocked embryos, indicating that cap recognition is not required for translation. In contrast, sickle mRNA is translated in a cap-dependent manner in all these assays. Our results show that IRES-dependent initiation may play a role in the translation of Drosophila proapoptotic genes and suggest a variety of regulatory pathways.
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Abbreviations
- cad :
-
caudal
- eIF:
-
eukaryotic initiation factor
- FLuc:
-
Firefly luciferase
- hid :
-
head involution defective
- hsp :
-
heat-shock protein
- IRES:
-
internal ribosome entry site
- PABP:
-
poly(A)-binding protein
- RLuc:
-
Renilla Luciferase
- rpr :
-
reaper
- skl :
-
sickle
- UTR:
-
untranslated region
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Acknowledgements
We thank A. Zechel for excellent technical assistance, R. Kühnlein for plasmid pRK27, J. M. Abrams for plasmid SK+II-Grim-short-cDNA, S. Höppner for proofreading the manuscript and M. Altmann for valuable comments on the manuscript. We also acknowledge the anonymous reviewers for constructive criticisms. This work was supported by the Max Planck Gesellschaft (MPG), the Deutsches Bundesministerium für Bildung und Forschung (grant 031U215B to R.R.-P.) and the University of La Plata. R.R.-P. is recipient of an International Partner Laboratory Program of the MPG in CREG.
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Vazquez-Pianzola, P., Hernández, G., Suter, B. et al. Different modes of translation for hid, grim and sickle mRNAs in Drosophila. Cell Death Differ 14, 286–295 (2007). https://doi.org/10.1038/sj.cdd.4401990
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DOI: https://doi.org/10.1038/sj.cdd.4401990
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