Abstract
A new cytotoxic indole-3-ethenamide (1) and two known compounds, 7-(3-methylbut-2-enyl)-1H-indole-3-carbaldehyde (2) and emodin (3) were isolated and identified from the ethyl acetate extract of Aspergillus sclerotiorum PT06-1 in a hypersaline nutrient-rich medium. On the basis of spectroscopic analysis and amino-acid analysis, the new structure of 1 was determined to be (S,E)-3-methyl-2-(N- methylacetamido)-N-(2-(7-(3-methylbut-2-enyl)-1H-indol-3-yl)vinyl)butanamide within 3:1 ratio of rotamers along the acetamido single bond in DMSO-d6 at room temperature. Compound 1 showed moderate cytotoxicity against A-549 cells and weak cytotoxicity against HL-60 cells with the IC50 values of 3.0 and 27 μM, respectively. Compound 2 has been separated as natural product for the first time, and its NMR data were also reported for the first time in this study.
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Acknowledgements
This work was supported by grants from the Major Program for Technique Development Research of New Drugs in China (No. 2009ZX09103-046), from Special Fund for Marine Scientific Research in the Public Interest of China (No. 2010418022-3), from National Basic Research Program of China (No. 2010CB833800), from the National Natural Science Foundation of China (No. 30470196 & 30670219), and from PCSIRT (No. IRT0944). The cytotoxicity assay was performed at the Shanghai Institute of Materia Medica, Chinese Academy of Sciences.
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Wang, H., Zheng, JK., Qu, HJ. et al. A new cytotoxic indole-3-ethenamide from the halotolerant fungus Aspergillus sclerotiorum PT06-1. J Antibiot 64, 679–681 (2011). https://doi.org/10.1038/ja.2011.63
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DOI: https://doi.org/10.1038/ja.2011.63
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