Fig. 3: Loading, viability and function of cells in multicompartment microcapsules.
From: Multicompartment hydrogel microcapsules for creating spatially patterned cell co-cultures
a Live/dead fluorescence image at day 0 and 5. b Quantification of cell viability at day 0 and 5. c Cell spheroid diameter increases over time in microcapsules (n = 3). d Urea production by encapsulated hepatic cells remains stable over time, pointing to hepatic phenotype maintenance (n = 3). e Albumin secretion increases over time, likely due to increasing cell number/spheroid size (n = 3). f Two groups of hepatic cells were labeled with either red or purple cell tracker dyes and then included in the core 1 and core 2 solutions. The image shows individual microcapsules carrying spatially distinct groups of red and purple cells at day 3 of culture. Data for (b), (c), (d), and (e) are presented as mean ± standard deviation. c, d and e are statistically evaluated by unpaired, one-tailed, equal-variance Student’s t test, with each group compared to the preceding experimental group for c and day 1 compared to day 3, 5, and 7 for (d) (NS: not significant, *p <0.05, **p <0.01, ***p <0.001, and ****p <0.001)
