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Use of autologous stem cells cryopreserved for over 15 years in stem cell transplantation for multiple myeloma

Bone Marrow Transplantation volume 56, pages 978–979 (2021)Cite this article

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It is standard practice in many centers to collect sufficient cells for more than one transplant [1] for multiple myeloma patients in order to use one aliquot for immediate transplantation and cryopreserve a second aliquot for the possible utilization as a salvage transplant in the future [2]. Patients regularly ask about the quality and integrity of previously collected stem cells. In this letter, we report on five patients that successfully received a salvage transplant over 15 years after the collection of their stem cells with prompt engraftment [3].

Peripheral blood leukapheresis was performed to collect mobilized hematopoetic progenitor cells (HPC). Processing and cryopreservation were performed under cGMP conditions (current good manufacturing practice). The cells were cryopreserved using a final nucleated cell concentration of 200–300 × 106 cell/ml depending on the year of collection with a final concentration of 10% DMSO using a controlled rate freezer. The cells were initially stored in liquid nitrogen freezer tanks until 2007 at which time the HPC were transfered to vapor phase tanks.

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Authors and Affiliations

  1. Division of Hematology, Mayo Clinic, Rochester, MN, USA

    Morie A. Gertz, Martha Q. Lacy & William J. Hogan

  2. Division of Transfusion Medicine, Mayo Clinic, Rochester, MN, USA

    Eapen K. Jacob

Authors
  1. Morie A. Gertz
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  2. Martha Q. Lacy
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  3. Eapen K. Jacob
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  4. William J. Hogan
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Correspondence to Morie A. Gertz.

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Conflict of interest

MAG reports personal fees from Ionis/Akcea, personal fees from Alnylam, personal fees from Prothena, personal fees from Janssen, grants and personal fees from Spectrum, personal fees from Annexon, personal fees from Appellis, personal fees from Amgen, personal fees from Medscape, personal fees from Physicians Education Resource, personal fees for Data Safety Monitoring board from Abbvie and Celgene, personal fees from Research to Practice, workforce training Sanofi, speaker fees from Teva, Speaker fees from Johnson and Johnson; Speaker fees from Medscape, Speaker fees DAVA oncology; Advisory Board for Pharmacyclics Advisory Board for Proclara; development of educational materials for i3Health. Educational Program development i3Health. Royalties from Springer Publishing. Grant Funding Amyloidosis Foundation; International Waldenstrom Foundation. NCI SPORE MM SPORE 5P50 CA186781-04. Stock Options Aurora Bio.

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Gertz, M.A., Lacy, M.Q., Jacob, E.K. et al. Use of autologous stem cells cryopreserved for over 15 years in stem cell transplantation for multiple myeloma. Bone Marrow Transplant 56, 978–979 (2021). https://doi.org/10.1038/s41409-020-01131-7

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