Fig. 1: MDM2 diminishes the stability of MEIS1 protein via the ubiquitin-proteasome degradation pathway. | Cell Death & Differentiation

Fig. 1: MDM2 diminishes the stability of MEIS1 protein via the ubiquitin-proteasome degradation pathway.

From: Ubiquitination of MEIS1 by MDM2 serves as a switch for p53 stabilization and DNA damage response activation

Fig. 1: MDM2 diminishes the stability of MEIS1 protein via the ubiquitin-proteasome degradation pathway.

A qRT-PCR assay was performed to measure the mRNA expression level of MEIS1 in paired samples of tumor and paired non-tumor tissues from CRC patients. B Western blot assay was conducted to determine the protein expression level of MEIS1 in paired samples of tumor and paired non-tumor tissues from CRC patients. C Venn diagram depicting the common E3 ligases predicted to mediate MEIS1 degradation, based on IP/MS analysis and data from Ubibrowser. D Western blot analysis of MEIS1 protein expression in cells transfected with MDM2 or HERC2. E Cells transfected with MDM2 were treated with MG132 to inhibit proteasomal degradation, and MEIS1 protein level was subsequently measured by western blot to assess the impact of MG132 on MEIS1 stability. F Cell lysates were subjected to IP/IB using specific antibodies against MEIS1 or MDM2, along with IgG as a control, to detect the endogenous interaction between MEIS1 and MDM2. G HEK293T cells were co-transfected with HA-Ub, Flag-MEIS1, MDM2 WT or MDM2 C464A, followed by MG132 treatment. Flag-tagged proteins were immunoprecipitated, and the ubiquitination status of MEIS1 was analyzed.

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