Fig. 3: MDM2 binding to the Ser/Thr-rich domain of MEIS1 promotes ubiquitination of MEIS1 at K178. | Cell Death & Differentiation

Fig. 3: MDM2 binding to the Ser/Thr-rich domain of MEIS1 promotes ubiquitination of MEIS1 at K178.

From: Ubiquitination of MEIS1 by MDM2 serves as a switch for p53 stabilization and DNA damage response activation

Fig. 3: MDM2 binding to the Ser/Thr-rich domain of MEIS1 promotes ubiquitination of MEIS1 at K178.

A MDM2 and its truncates were co-transfected with MEIS1 into HEK293T cells, followed by detection of the binding between MEIS1 and the MDM2 domain. MEIS1 and its truncates were co-transfected with MDM2 into HEK293T cells, followed by detection of MDM2 binding to the MEIS1 domain (B) or the ubiquitination level of MEIS1 (C). D MEIS1-WT, K72R, K75R, K93R, K126R, K132R, K162R, K178R or K180R were co-transfected with Flag-MDM2 into HEK293T cells and treated with MG132. The ubiquitination level of MEIS1 was detected. E Schematic representation of MEIS1 and MDM2 binding domains.

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