Fig. 5: The regulatory domain of p53 is combined with the Asp/Glu-rich domain of MEIS1. | Cell Death & Differentiation

Fig. 5: The regulatory domain of p53 is combined with the Asp/Glu-rich domain of MEIS1.

From: Ubiquitination of MEIS1 by MDM2 serves as a switch for p53 stabilization and DNA damage response activation

Fig. 5: The regulatory domain of p53 is combined with the Asp/Glu-rich domain of MEIS1.

A The interaction of endogenous p53 and MDM2 was detected by IP/IB assays in cells with MEIS1 overexpression. B The interaction of endogenous MEIS1 and MDM2 was detected by IP/IB assays in cells with p53 overexpression. C The interaction of endogenous p53 and MEIS1 was detected by IP/IB assays in cells with p53 overexpression. D HEK293T cells were co-transfected with Flag-MEIS1 (or no-tag MEIS1 as control), p53 and MDM2 then treated with MG132. Two-step co-immunoprecipitation identifies a MEIS1-p53-MDM2 complex. The procedure is outlined in the box at the top. E MEIS1 and its truncates were co-transfected with p53 into HEK293T cells, followed by detection of the binding between p53 and the MEIS1 domain. F p53 and its truncates were co-transfected with MEIS1 into HEK293T cells, followed by detection of the binding between MEIS1 and the p53 domain. G Schematic representation of MEIS1, p53 and MDM2 binding domains.

Back to article page