Fig. 2: BCKDK regulates BCAA transporter expression in GBM via IGF2BP3. | Cell Death & Differentiation

Fig. 2: BCKDK regulates BCAA transporter expression in GBM via IGF2BP3.

From: Targeting the PSMD14-BCKDK pathway overcomes immune suppression and enhances CAR-NK infiltration in glioblastoma

Fig. 2: BCKDK regulates BCAA transporter expression in GBM via IGF2BP3.

RT-PCR analysis showing reduced SLC7A5 and SLC7A8 mRNA levels after BCKDK inhibition (A) or knockdown (B) in GBM cells. All data are mean ± SEM. P values were calculated using an unpaired two-tailed Student’s t test. C, D IF showing decreased SLC7A5/SLC7A8 protein levels following BCKDK inhibition or knockdown in A172 cells, scale bars: 100 μm. E, F IP and WB showing BCKDK-dependent threonine phosphorylation of IGF2BP3. G, H WB and RT-qPCR showing reduced SLC7A5/SLC7A8 expression following IGF2BP3 knockdown in A172 and U251 cells. I A172 cells were transfected stably with scramble shRNA and IGF2BP3 shRNA, and were treated with actinomycin D (20 μg/mL) for the indicated time, and SLC7A5 and SLC7A8 mRNA levels were analyzed.

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