Fig. 4: Transcriptomic profiling of Jmjd7KO vs. WT osteoblasts.

Principal component analysis showing distinct clustering of gene expression profiles (a). Volcano plot depicting 519 differentially expressed genes (b), with corresponding heatmap of changes (c). Gene ontology analysis reveals enrichment in osteoblast differentiation, bone mineralization, chromatin remodeling, glycolysis, and cytokine signaling (d). KEGG pathway analysis indicates involvement in carbon metabolism, fatty acid metabolism, and OXPHOS (e). GESA demonstrates reduced mitochondrial metabolic activity in Jmjd7KO osteoblasts (f). RNA sequencing was performed on osteoblasts from three Jmjd7KO and WT mice.