Fig. 1: Loss of lymphatics in shoc2 mutant zebrafish.
From: Signaling scaffold Shoc2 regulates lymphangiogenesis by suppressing mTORC1-mediated IFN responses
A Brightfield image of a lateral view of the entire zebrafish larvae of wild-type sibling (top panel) and shoc2SA/SA mutant (bottom panel). The red arrow is pointing to an edematous site. B Brightfield images of a dorsal view of the entire zebrafish larvae of wild-type sibling (top panel) and shoc2SA/SA mutant (bottom panel). Red arrows are pointing to edematous sites. C Schematic of areas of confocal imaging highlighting the trunk and craniofacial vessels. D Confocal image of trunk vessels of wild-type sibling Tg(mrc1a:eGFP)y251; Tg(kdrl:mCherry)y171 zebrafish larvae at 5 dpf. Scale bar: 100 μm. TD, thoracic duct; ISLV, intersegmental lymphatic vessel; DLLV, dorsal longitudinal lymphatic vessel. E Confocal image of trunk vessels of a wild-type sibling Tg(mrc1a:eGFP)y251 showing a separated green channel from (D). Scale bar: 100 μm. F Confocal image of trunk vessels of a wild-type sibling Tg(kdrl:mCherry)y171 showing a separated red channel from(D). Scale bar: 100 μm. G Confocal image of trunk vessels of a shoc2SA/SA mutant Tg(mrc1a:eGFP)y251; Tg(kdrl:mCherry)y171 zebrafish larvae at 5 dpf. Asterisks indicate the absence of lymphatic vessels. Scale bar: 100 μm. H Confocal image of trunk vessels of a shoc2SA/SA mutant Tg(mrc1a:eGFP)y251 showing separated green channel from (D). Scale bar: 100 μm. I Confocal image of trunk vessels of a shoc2SA/SA mutant Tg(kdrl:mCherry)y171 showing a separated red channel from (D). Scale bar: 100 μm. J Quantitation of the number of somites with TD segments in wild-type or shoc2SA/SA mutants at 5 dpf (n = 6 larvae in each group). K Quantitation of the number of somites with DLLV segments in wild-type or shoc2SA/SA mutants at 5 dpf (n = 6 larvae in each group). L Quantitation of the number of somites with ISLV in wild-type or shoc2SA/SA mutants at 5 dpf (n = 6 larvae in each group). M Confocal image of the craniofacial lymphatics of a wild-type sibling at 5 dpf. LFL lateral facial lymphatics, MFL medial facial lymphatics, OLV otolithic lymphatic vessel, LAA lymphatic branchial arches. Scale bar: 100 μm. N Confocal image of the craniofacial lymphatics of a shoc2SA/SA mutant at 5 dpf. Asterisks indicate the absence of lymphatic vessels. Scale bar: 100 μm.
