Fig. 3: Identification of Fer2 transcriptional targets through a combination of ChIP-seq and RNA-seq approaches. | Nature Communications

Fig. 3: Identification of Fer2 transcriptional targets through a combination of ChIP-seq and RNA-seq approaches.

From: Maintenance of mitochondrial integrity in midbrain dopaminergic neurons governed by a conserved developmental transcription factor

Fig. 3: Identification of Fer2 transcriptional targets through a combination of ChIP-seq and RNA-seq approaches.

a ChIP-seq was performed on 14-day-old Fer2::GFP::V5, Fer21 flies. w1118 flies were used as a negative control. n = 2 biological replicates per group. b A sequence containing an E-box (CANNTG, underlined) was found enriched by Fisher’s exact test in FER2 ChIP-seq peaks over w1118 ChIP-seq. c Scheme of the whole-head RNA-seq analysis to identify DEG after heat shock-driven Fer2 transient overexpression. n = 3 biological replicates per group. The number of DEGs up- and downregulated after heat shock are shown. d Examples of ChIP-seq and RNA-seq data (48 h) for two Fer2 direct targets, CG8128 and skd. The rectangles indicate ChIP-seq peaks.

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