Fig. 2: Global changes in contact domain boundaries in RBLs and LCLs.
From: A DNA tumor virus globally reprograms host 3D genome architecture to achieve immortal growth
a Venn diagram representing the contact domain boundaries from Hi-C in RBLs and LCLs. b ChIP-seq signals for CTCF in LCLs and RBLs, EBNALP and EBNA3A were plotted along the edges of LCL unique boundaries (centered on CTCF, +/− 2 kb). Left and right indicate the relative edge positions of the contact domain boundaries. c. ChIP-seq signals for CTCF in RBLs and LCLs plotted along the edges of RBL unique boundaries (centered on CTCF, +/−2kb). d Representative contact domain changes due to EBV transformation at chromosome 2, 153 Mb – 159 Mb. Top: ChIP-seq tracks and contact frequencies of interactions in LCLs. Bottom: ChIP-seq tracks and contact frequencies of interactions in RBLs. Highlighted in red squares are CTCF profiles that changed at contact domain boundaries between the two conditions. Heatmaps are colored based on normalized interaction frequencies at 25 kb resolution shown at the bottom. e Cumulative distal to local interaction (DLR) values at 25 kb resolution near TSSs of genes that are essential for LCL growth and survival (solid lines) and all annotated genes (dotted lines) in both RBLs (orange) and LCLs (teal).
