Fig. 4: ESEs and their direct target interactions in RBL to LCL time course.
From: A DNA tumor virus globally reprograms host 3D genome architecture to achieve immortal growth
a RBLs (day 0) were transformed into LCLs (day 28) in a time course experiment in replicates. ESE linkages to their direct genes were evaluated by POLR2A HiChIP. Each magenta line represents a genomic interaction. HiChIP links with filter threshold >2 are shown. HiChIP loops in RBLs and LCLs at a. ATP1A1 and CD58, b BUB3. RBL and LCL H3K27ac, CTCF and LCL RAD21, SMC3 ChIP-seq tracks are also shown on the top. c RBLs and LCLs 28 days post infection were fixed and hybridized with labeled BACmids. The nuclei were stained with DAPI. The BAC probes used are indicated in panel b. More than 50 nuclei were scored for each cell type and tabulated on the right. Pearson’s Chi-square test P < 3.69-e-9. Source data are provided as a supplementary Source Data file.
