Fig. 3: 3D chromatin structure of the Kit locus in MEFs.
From: TAD border deletion at the Kit locus causes tissue-specific ectopic activation of a neighboring gene
CBSs deletions Pdgfra Δ2k and Pdgfra Δ60k were insufficient to disrupt the TADs insulation between Pdgfra and Kit in MEFs, as demonstrated by comparison of the wild-type (Wt) (a), Pdgfra Δ2k mutant (b) and Pdgfra Δ60k mutant (d) Hi-C heatmaps (5 kb resolution). Colour bar reflects the interaction counts. Ectopic contacts in Pdgfra Δ60k are indicated by arrows. Spatial contact enrichment between TADs is additionally visualized by subtraction maps (10 kb resolution) for Pdgfra Δ2k (c) and Pdgfra Δ60k (e) relative to Wt. Gain of contacts in mutants is indicated in red, and loss of contacts in blue. The tracks below the subtraction maps show the estimated contact enrichment. f RNA-seq, CTCF and H3K27ac ChIP-seq signals across the Kit locus.
