Fig. 4: Comparison of the TADs disruption at the Kit locus in mast cells and melanocytes.
From: TAD border deletion at the Kit locus causes tissue-specific ectopic activation of a neighboring gene
a Cytospin preparations of Wt (left) and Kit Δ30k (right) mast cells after 4 weeks in culture. Histamine containing granules could be detected by toluidine blue staining. Scale bar: 100 um; b Primary Wt (left) and Kit Δ30k (right) melanocytes after 2.5 weeks in culture (without staining). Scale bar: 100 um. 3D genome organization and regulation context in Wt and Kit Δ30k mast cells (c) and melanocytes (d) are presented by Hi-C maps (5 kb resolution), CTCF and H3K27ac ChIP-seq, and RNA-seq. Colour bars reflect the interaction counts. Spatial contacts newly established after the TADs disruption are indicated by arrows. Subtraction maps demonstrate changes of spatial contact frequencies in mutant mast cells (e) and melanocytes (f) relative to the Wt. The tracks below the subtraction maps show the estimated contact enrichment, indicating loss of insulation in the mutant cells. Removal of the Kit/Kdr boundary resulted in loss of inter-TAD insulation and extensive interactions across the TADs boundary.
