Fig. 5: Kit/Kdr TADs fusion induces Kdr activation in melanocytes.
From: TAD border deletion at the Kit locus causes tissue-specific ectopic activation of a neighboring gene
a Coat color difference between brown Kit Δ30k (left) and black Wt (right) mice; b A plot of normalized RNA-seq reads counts in Wt and Kit Δ30k melanocytes indicates a 6.7-fold increase of Kdr transcription. Dots represent individual replicates. p-value was estimated with DESeq2, using the two-sided Wald test; c In mast cells Kit Δ300k deletion caused an antisense Kdr transcription; d Crossing scheme used to obtain Mus musculus (black) x Mus castaneus (orange) hybrids and further RNA-seq analysis; e Expression relative to a M. castaneus (Cast) allele. To evaluate an allelic activity, reads with M. castaneus-specific SNPs were scored for Pdgfra (n = 19 SNPs), Kit (n = 12 SNPs) and Kdr (n = 21 SNPs). Data are presented as mean values  ±  SD. p value was estimated using two-tailed Mann–Whitney test, NS — not significant. Source data are provided as a Source Data file.
