Fig. 4: The ACR-11/nicotinic α7 receptor cell autonomously mediates the UPR^mt.

a (i) Structure of the acr-11 locus. rp191 = 3038 bp deletion in wild-type animals; rp192 = 3046 bp deletion in ace-2(g72); ace-1(p1000) animals. (ii) Structure of the acr-6 locus. rp209 = 3209 bp deletion in wild-type animals; rp210 = 3158 bp deletion in acr-11(rp191) animals. (iii) Structure of the acr-7 locus. rp212 = 1556 bp deletion. Black boxes, coding regions; grey boxes, untranslated regions; red lines, CRISPR/Cas9-generated deletion alleles. b Quantification of UPR^mt reporter (hsp-6p::gfp) expression in L4 larvae of wild-type, acr-11(rp191), ace-2(g72); ace-1(p1000), and ace-2(g72) acr-11(rp192); ace-1(p1000) animals. c Quantification of UPR^mt reporter (hsp-6p::gfp) expression in L4 larvae of wild-type, acr-6(rp209), acr-7(rp212), acr-11(rp191), acr-6(rp209); acr-7(rp212), acr-6(rp210) acr-11(rp191), acr-11(rp191); acr-7(rp212) and acr-6(rp210) acr-11(rp191); acr-7(rp212) animals. d, e Quantification (d) and DIC/fluorescent micrographs (e) of UPR^mt reporter (hsp-6p::gfp) expression in L4 larvae of wild-type, ace-2(g72); ace-1(p1000), acr-11(rp191), ace-2(g72) acr-11(rp192); ace-1(p1000) and ace-2(g72) acr-11(rp192); ace-1(p1000); ges-1p::acr-11 DNA animals. f, g Quantification (f) and DIC/fluorescent micrographs (g) of UPR^mt reporter (hsp-6p::gfp) expression in L4 larvae of wild-type, unc-17(e113), ges-1p::acr-11 DNA and unc-17(e113); ges-1p::acr-11 cDNA animals. n = 30 animals. P values assessed by one-way analysis of variance (ANOVA) with Tukey’s post hoc test. Error bars indicate SEM. Scale bars, 250 µm. Source data are provided as a Source Data file.