Fig. 6: Demonstration of multi-photon bio-imaging of cells with Au3-CD films.
a Schematic illustration of multi-photon bio-imaging of J774A.1 by employing Au3-CD film. b TEM images for the cellular active uptake process of Au3 NPs by J774A.1 macrophage. The black arrows and yellow arrows indicate the HPβCD enveloped Au3 NPs and pseudopodia, respectively. c Enlarged TEM image for Au3 NPs in the cytoplasm of J774A.1. The red arrows indicate representative Au3 NPs. d CLSM images of J774A.1 incubated with Au3-CD film for 2.5 h. e CLSM images of E. coli incubated with Au3-CD film for 12 h. CLSM images were captured under bright-field (left), 850 nm laser irradiation (middle) and merged mode (right). The MPEL images captured at 850 nm excitation and 550–750 nm emission are pseudo-colored for better distinction.
