Fig. 1: Structure of LAG3 bound to MHC class II.
From: Structural basis for mouse LAG3 interactions with the MHC class II molecule I-Ab
a Cartoon depicting LAG3 (cyan) in the context of an MHC-II (violet/pink) bound to the TCR-CD3 complex (grey). D1 of LAG3 binds to MHC-II, and D2 mediates LAG3 dimerization. b Schematic of the scLAG3-MHC-II construct used for structure determination. c A size-exclusion chromatogram from purification of scLAG3-MHC-II indicates that the construct elutes as a monodisperse peak. The inset SDS-PAGE gel shows the purified LAG3D12-MHC-IIα and CLIP-MHC-II β single-chain fusion proteins. This data is representative from one of at least three independent scLAG3-MHC-II purifications. Source data are provided as a Source Data file. d Crystal structure of the scLAG3-MHC-II construct consisting of the LAG3 D1 and D2 domains (teal) bound to I-Ab (violet/salmon) loaded with CLIP (yellow).
