Fig. 4: The MHC-II-specific antibody, 44H10, disrupts MHC-II binding to LAG3 and CD4.
From: Structural basis for mouse LAG3 interactions with the MHC class II molecule I-Ab
a Structural alignments showing that the antibody 44H10 (blue)34 occupies the MHC-II interface bound by LAG3 (cyan, left) and CD4 (yellow, right). b Raji APC cells were stained with a fluorescently labeled LAG3 tetramers (20 nM) in the presence or absence of a 44H10 single-chain variable fragment (scFv)-Fc fusion protein. The control represents Raji APC cells incubated with streptavidin-647 alone. Data shown as normalized median MFI ± SEM based on triplicate wells from one representative experiment. The experiment was independently repeated two times. Statistics were obtained using a one-way ANOVA multiple comparison in Prism 9 (Version 9.5.1). c BLI sensograms depict the binding of immobilized human LAG3 protein to HLA-DR4HIV in the presence of increasing concentrations of 44H10 scFv-Fc fusion protein. d BLI sensograms depict the binding of immobilized CD4HA protein to HLA-DR4HIV in the presence of increasing concentrations of 44H10 scFv-Fc fusion protein. The plots in c and d each depict a representative sensogram from two independent experiments. Source data are provided as a Source Data file.
