Fig. 6: Comparison of the MHC-II- and FGL1-binding sites of LAG3.
From: Structural basis for mouse LAG3 interactions with the MHC class II molecule I-Ab
a Mutations known to affect LAG3 binding to FGL1 (blue) were mapped onto the structure of scLAG3-MHC-II. LAG3 is shown in gray surface representation, and a boundary surrounding the putative FGL1-binding residues is shown as a dotted line. b Raji cells were stained with fluorescently labeled LAG3 tetramers (50 nM) in the presence of the FGL1 fibrinogen-like domain fused to the C-terminus of human IgG1 Fc (FGL1FD-Fc) or IgG1 Fc. Background-subtracted median MFI was normalized to Fc control. Data shown as normalized median MFI ± SEM based on triplicate wells from one representative experiment. The negative control shows Raji cells stained with Streptavidin 647 (SA-647) alone. Statistics were obtained using a one-way ANOVA multiple comparison in Prism 9 (Version 9.5.1). A representative data plot from one of two independent experiments is shown. Source data are provided as a Source Data file.
