Fig. 3: Influence of GADs on the channel gates and glutamate permeability.
From: GAD65 tunes the functions of Best1 as a GABA receptor and a neurotransmitter conducting channel
a, b I-V relationships of Best1-3A alone (black), Best1-3A + GAD65 (red solid) and Best1-3A + GAD67 (red open), with Cl− (a) or glutamate (b) as the principal anion in both sides of patch solutions; n = 5-6. *p < 0.05 compared to Best1-3A alone. c, d I-V relationships (c) and PGlu/PCl relative permeability ratios (d) with external glutamate and internal Cl− in Best1-3A alone (black), Best1-3A + GAD65 (red solid) and Best1-3A + GAD67 (red open); n = 5. *p < 0.05 compared to Best1-3A alone. e–h The same format as (a–d) for Best1-I205A; n = 5–7; *p < 0.05 compared to Best1-I205A alone. All experiments are done at 1 μM [Ca2+]i in HEK293 cells. All error bars are presented as mean values +/- SEM; p values are calculated by two-tailed unpaired Student’s t test.
