Fig. 4: Role of the Best1 C-terminus in glutamate permeability and interaction with GADs.
From: GAD65 tunes the functions of Best1 as a GABA receptor and a neurotransmitter conducting channel
a I-V relationships of Best11-405 alone (black), Best11-405 + GAD65 (red solid) and Best11-405 + GAD67 (red open), with Cl− as the principal anion in both sides of patch solutions; n = 6–15. b Co-expressed Best11-405-Ven-His and GAD-Cer-Myc were examined by immunoblotting in input (top) and immunoprecipitation (IP, bottom) samples. The experiment was biologically replicated three times with similar results, and representative blots are shown. Original scans are provided in Supplementary Fig. 1a. c, d Bar chart showing the steady-state current densities at +100 mV from cells transiently expressing the indicated Best1 C-terminal truncation constructs alone (black) and in the presence of GAD65 (c, red) or GAD67 (d, red) with Cl− as the principal anion in both sides of patch solutions; n = 5–15. e The same format as (c), but with glutamate as the principal anion in both sides of patch solutions; n = 5–6. All experiments are done at 1 μM [Ca2+]i in HEK293 cells. All error bars are presented as mean values +/− SEM.
