Fig. 5: Drosophila Mettl1 is required to maintain tRNA abundance in the testis.

a Volcano plot representing changes in tRNA abundance between Mettl1-KO (Mettl1^KO1) and wild-type (yw) testes. Each dot shows the change in abundance of one tRNA. The two-sided P-values were calculated by applying the Wald test with the Benjamini-Hochberg correction implemented in DEseq2 module of the tRAX pipeline. b Heatmap showing changes in m⁷G-modified tRNA abundance between Mettl1-KO (Mettl1^KO1) and WT testis. Drosophila tRNAs were classified into three groups according to the difference in abundance between Mettl1-KO (Mettl1^KO1) and wild-type (WT), and P-values indicate significant differences; group 1 (m⁷G-modified tRNAs and significantly decreased abundance, log2FC < 0, P < 0.05); group 2 (other m⁷G-modified tRNAs, P ≥ 0.05); group 3 (non-m⁷G tRNAs). The P-values indicated correspond to those of (a). c Quantitative comparison of changes in tRNA abundance between the three groups of tRNAs (Group1: n = 15, Group2: n = 8, Group3: n = 22) defined in this study. Two-sided P-values were calculated by the Mann–Whitney U-test are indicated. Box plots represent maximum, median and minimum values with outliers. Central bands, upper and lower edges of box plots represent median, first and third quartiles, respectively. Upper and lower whiskers represent maximum, and minimum values. In our tRNA expression analysis, 3 biological replicates were set for each sample. d Northern blot analysis of tRNA abundance in Mettl1-KO (Mettl1^KO1) and WT (yw) testes. 2S rRNA was used as a loading control. Each northern blot was reproduced for three times. Source data of Fig. 5 are provided as a Source Data file.