Fig. 2: Gene drive spread in cell culture.
From: Viral gene drive spread during herpes simplex virus 1 infection in mice
a Viral titers in the supernatant after infection of N2a cells with WT, GD, GD-ns or GD-ΔCas9. Cells were infected with a single virus at MOI = 1. n = 4. b, c Viral titers in the supernatant after co-infection of N2a cells with WT + GD, WT + GD-ns or WT + GD-ΔCas9, with a starting proportion of gene drive virus of 20% (b) or 40% (c). MOI = 1, n = 4. d–g Evolution of the viral population after co-infection with WT + GD, WT + GD-ns or WT + GD-ΔCas9, with a starting proportion of gene drive virus of 20% (d, e) or 40% (f, g). Panels (d and f) show the proportion of viruses expressing mCherry, representing gene drive virus. Panels (e and g) show the proportion of viruses expressing the different fluorophore combinations. Viral titers are expressed in log-transformed PFU (plaque-forming unit) per mL of supernatant. Error bars represent the standard error of the mean (SEM) between biological replicates. n = 4. Source data are provided as a Source Data file.
