Fig. 3: Gene drive spread during herpes simplex encephalitis.
From: Viral gene drive spread during herpes simplex virus 1 infection in mice
a Infection routes along the optic, oculomotor and trigeminal nerves (cranial nerves II, III and V, respectively) following ocular inoculation of HSV-1. Male and female Balb/c mice were infected with 106 PFU in the left eye. b, c Viral titers after four days in the eye, TG and whole brain after (b) infection with a single virus, n = 5 mice, or (c) with a starting proportion of gene drive virus of 15%, n = 6 mice. d, e Viral population in the eye, TG and whole brain after co-infection with WT + GD or WT + GD-ns, after four days. n = 6. f Proportion of viral genomes with a mutated target site in the brain after four days. n = 3. g Viral titers in the spinal cord and brain after inoculation of WT, WT + GD, or WT + GD-ns in the right hind leg footpad, after 5–7 days. n = 8 for WT and WT + GD, n = 4 for WT + GD-ns. h, i Viral population in the spinal cord and whole brain after co-infection with WT + GD or WT + GD-ns, after 5–7 days. n = 5 for WT + GD, n = 1 for WT + GD-ns. Viral titers are expressed in log-transformed PFU. In panels (b, c and g) black lines indicate the median. n.d.: non-detected. Panels (d, e, f, h, and i) show the average and SEM between biological replicates. Source data are provided as a Source Data file.
