Fig. 6: High levels of co-infection in the brain during HSV-1 infection.
From: Viral gene drive spread during herpes simplex virus 1 infection in mice
a Images of brain sections were collected in three regions in the thalamus, midbrain and brain stem after ocular infection. b YFP, CFP and RFP cellular intensity after machine learning-assisted cell segmentation of brain sections. Datapoints were colored by converting YFP, CFP and RFP signals into the CYMK color space. 10,028 cells were detected, originating from 95 images and n = 3 mice. c Percentage of infected cells expressing one, two, or three fluorescent markers, both in the whole brain and in specific subregions. n = 3 mice. Data show the average and SD between biological replicates. d Representative images of the brain in the thalamus (sections S1), midbrain (sections S2) and brain stem (section S3), from three biological replicates. e–h Representative images and summary of co-infection patterns in specific subregions. LGN: lateral geniculate nucleus; SC: superior colliculus; EW: Edinger–Westphal nucleus; TGN: trigeminal nerve nuclei. Scale bars: 100 μm. Source data are provided in Supplementary data 2 and as a Source Data file.
