Fig. 8: Gene drive spread during latent infection in Swiss-Webster mice.
From: Viral gene drive spread during herpes simplex virus 1 infection in mice
a Experimental outline: Swiss-Webster mice were infected with 105 PFU of HSV1-WT on both eyes after corneal scarification. Four weeks later, mice were superinfected with 107 PFU of GD or GD-ns on both eyes, after corneal scarification. Another four weeks later, latent HSV-1 was reactivated twice with JQ1, two weeks apart. n = 14 mice per group. b Titer and number of shedding events in eye swabs on days 1–3 following JQ1 treatment, by qPCR. Shedding events from the same mouse are connected by a line. c Genotyping of positive eye swabs from five mice, using two duplex ddPCR assays. The first assay detected and quantified mCherry levels. The second assay distinguished between YFP and CFP. n = 8. d Number and proportion of TG and mice with detectable CFP. e Latent viral load in the TG by duplex ddPCR, detecting mCherry, YFP, CFP markers, or all HSV sequences. n = 28. f Proportion of CFP and mCherry in the TG. n = 28. Titers are expressed in log-transformed copies per swab, or per million cells after normalization with mouse RPP30 levels. Black lines indicate the median. n.d.: non-detected. Source data are provided as a Source Data file.
