Table 4 Primary and Secondary Antibody table
Antibody | Manufacturer | Catalog # | Host | Dilution |
|---|---|---|---|---|
Primary Antibodies | ||||
Human Nuclear Antigen (hNA) | Abcam | Ab191181 | Mouse | 1:50 |
IBA1 | Fujifilm-Wako | 019-19741 | Rabbit | 1:500 |
Ku80 | Abcam | Ab80592 | Rabbit | 1:250 |
CD163 | R&D Systems | AF1607 | Goat | 1:200 |
CD45 | BioLegend | 304002 | Mouse | 1:50 |
TMEM119 | Abcam | Ab185333 | Rabbit | 1:400 |
PU.1 | Cell Signaling | 2258S | Rabbit | 1:200 |
Ki67 | Agilent Technologies | M724029-2 | Mouse | 1:100 |
CD68 | Bio-Rad | MCA1957 | Rat | 1:100 |
P2RY12 | Sigma Aldrich | HPA014518 | Rabbit | 1:250 |
TREM2 | R&D Systems | AF1828 | Goat | 10ug/mL |
STEM121 | Takara | Y40410 | Mouse | 1:200 |
SALL1 | Thermo Fisher Sc. | PA5-62057 | Rabbit | 1:200 |
LAMP1 | Cell Signaling | 9091 T | Rabbit | 1:200 |
SGSH (WB) | Bio-Techne | MAB83801 | Mouse | 20ug/ml |
Secondary Antibodies | ||||
Anti-Goat IgG AlexaFluor 488 | Invitrogen | A-21202 | Donkey | 1:500 |
Anti-Mouse IgG AlexaFluor 555 | Invitrogen | A-31570 | Donkey | 1:500 |
Anti-Rabbit IgG AlexaFluor 555 | Invitrogen | A-31572 | Donkey | 1:500 |
Anti-Mouse IgG AlexaFluor 647 | Invitrogen | A-32787 | Donkey | 1:500 |
Anti-Rabbit IgG AlexaFluor 647 | Invitrogen | A-31573 | Donkey | 1:500 |
Anti-Rat IgG AlexaFluor 488 | Invitrogen | A-21208 | Donkey | 1:500 |
Biotin-SP Anti-Rabbit IgG | Jackson ImmunoResearch Laboratories | 711-065-152 | Donkey | 1:500 |
Peroxidase Streptavidin-HRP | Jackson ImmunoResearch Laboratories | 016-030-084 | 1:250 | |
Anti-Mouse HRP (WB) | Bio-Techne | 042-205 | ||
