Fig. 7: Aβ42 fibrils can seed wact-190 objects.
From: A rapid in vivo pipeline to identify small molecule inhibitors of amyloid aggregation
A–D Transmission electron microscopy (TEM) images of solutions allowed to self-assemble overnight. A–D Mixing solutions of 25 μM Aβ42 (A) and 25 μM wact-190 (B) in PBS in either a 1:2 (C) or 1:10 (D) ratio, respectively. E–H Mixing solutions of 25 μM Aβ42 (E) and 0.5 μM wact-190 (F) in PBS in a 1:1 ratio (G, H). I–L Mixing solutions of 25 μM Aβ42 (I) and 0.1 μM wact-190 (J) in PBS in a 1:1 ratio (K, L). Three independent trials of experiments shown in (A–L) were performed with similar results. M–P A second trial of mixing solutions of 25 μM Aβ42 (M) and 25 μM wact-190 (N) in PBS in a 1:1 ratio (O, P). A total of 3 trials (N = 3) of this type were performed. Q–X Transmission electron microscopy (TEM) images of solutions allowed to self-assemble over 6 days. Only one independent trial of the 6-day assays were performed (N = 1), but it is corroborated by the overnight assays. Q–S Mixing solutions of 25 μM Aβ42 (Q) and 25 μM wact-190 (B) in PBS in a 1:10 ratio (S). U–W Mixing solutions of 25 μM Aβ42 (U) and 25 μM wact-11 (V) in PBS in a 1:10 ratio (W) shows a lack of objects associated with amyloid fibrils. T, X show PBS-only mock samples. In all panels, examples of Aβ42 amyloid fibrils are indicated with blue arrows and examples of presumptive wact-190 objects that are associated with Aβ42 fibrils are indicated with red arrows.
