Fig. 5: SOLID enables whole-brain profiling of diverse neural projections labeled with AAVs.
From: SOLID: minimizing tissue distortion for brain-wide profiling of diverse architectures
A 3D reconstruction of AAV-retro-labeled afferent projections (green) to pontine nuclei (PN) throughout the brain. B 3D reconstruction of AAV-labeled antegrade projections (magenta) from PN. C The counterstained cell nuclei channel (cyan). D Reconstruction of the merged channel images from (A–C) (from 2 replicates). E Sagittal view of the 3D reconstruction shown in (D). F Registered brain section images for the retrograde projection channel. G Registered brain section images for the antegrade projection channel. H Horizontal and sagittal views of the brain-wide neural projections to PN with standard anatomical annotations. I Horizontal and sagittal views of the brain-wide neural projections from PN with standard anatomical annotations. J Quantification of the relative intensity of retrograde projections to PN in different brain regions. K Quantification of the relative intensity of antegrade projections from PN in different brain regions. Illustration of the mapped neural projections are displayed in accordance with Allen CCF border lines and region-based color-coding. Source data are provided as a Source Data file.
