Fig. 6: SOLID realizes cross-channel investigation of brain-wide pathological changes during the progression of AD.

A Three-channel rendering of neurons (green), blood vessels (glow) and Aβ plaques (blue) (from >3 replicates). B Magnification of the boxed region in (A). C Registration of the multi-color brain datasets to ABA. D 3D rendering of the segmented data blocks for indicated brain regions. E Representative 3D rendering for Aβ plaque and blood vessel data blocks within the FRP region in normal, 3 M and 8 M AD mice. F Quantitative analysis of Aβ plaque density and vessel length density in 83 sub-regions in 3 M and 8 M AD mice. To calculate fold-changes for Aβ plaque density and decrease rate for vessel length density, mean values of calculated data for each age are used (n = 5 samples for AD 3 M, n = 7 samples for AD 8 M). G Quantitative analysis of total number and density of vessel-related Aβ plaques in 11 major brain regions in 3 M and 8 M AD mice (n = 5 samples for AD 3 M, n = 7 samples for AD 8 M). H Representative 3D rendering for the identified Aβ plaques, neuronal somata and neuronal somata invaded by Aβ plaques within the SSs region in 3 M and 8 M AD mice. Different colors represent different layers of the cortex. I Quantification of the Aβ plaque/neuronal somata overlap ratio in different layers of the cerebral cortex in 3 M and 8 M AD mice. To calculate the overlap ratio, mean values of Aβ plaque/neuronal somata for each group are used. (n = 5 samples for AD 3 M, n = 7 samples for AD 8 M). All values are presented as the mean ± SD. Source data are provided as a Source Data file.