Fig. 2: Comparison of the nuclease activities between the wild-type Cas12a and split Cas12a RNPs.
From: Split crRNA with CRISPR-Cas12a enabling highly sensitive and multiplexed detection of RNA and DNA
a Cis-cleavage of the target dsDNA plasmids by wild-type Cas12a RNPs (blue) and split Cas12a RNPs (orange). The reactions contained 50 nM Cas12a, 100 nM crRNA (or 100 nM split RNA), and 10 nM target dsDNA plasmids. b Trans-cleavage of ssDNA probes by wild-type Cas12a RNPs (blue) and split Cas12a RNPs (orange) in the presence of target or non-target dsDNA substrates. The reactions contained 50 nM Cas12a, 100 nM crRNA (or 100 nM split RNA),10 nM complementary dsDNA activators (or 10 nM non-target dsDNA), as well as 20 nM ssDNA probes. c Trans-cleavage of ssDNA probes by wild-type Cas12a RNPs (blue) and split Cas12a RNPs (orange) in the presence of target or non-target ssDNA substrates. The reactions contained 50 nM Cas12a, 100 nM crRNA (or 100 nM split RNA),10 nM complementary ssDNA activators (or 10 nM non-target ssDNA), as well as 20 nM ssDNA probes. For a to c, all the experiments were performed three times.
